RCurl作者 Duncan Temple Lang 现任加州大学 U.C. Davis分校副教授 致力于借助统计整合进行信息技术的探索 RCurl的概述 The RCurl package is an R-interface to the libcurl library that provides HTTP facilities. This allows us to download files from Web servers, post forms, use HTTPS (the secure HTTP), use persistent connections, upload files, use binary content, handle redirects, password authentication, etc. RCurl这个程序包提供了由R到libcurl库的接口,从而实现HTTP的一些功能。例如,从 服务器下载文件、保持连接、上传文件、采用

principle: 对于 数字 命名的染色体号； 默认人类编号： 23/X； 24/Y； 25/XY； 26/MT； 所以27会报错； solve: --chr-set --autosome-num --dog/sheep/horse/mouse

locate libpcap cd /usr/lib/ sudo ln -s libpcap.so libpcap.so.0.8

bwa aln -l 1024 ref.fa reads1.fq > aln_sa1.sai bwa aln -l 1024 ref.fa reads2.fq > aln_sa2.sai bwa sampe reference.fa aln_sa1.sai aln_sa2.sai reads1.fq reads2.fq > aligned_pairs.sam bwa aln -l 1024 ref.fa reads.fq > aln_sa.sai bwa samse reference.fa aln_sa.sai reads.fq > aligned_se.sam

使用 python 语法高亮 后缀，Snakefile .smk .rules 自动高亮

链接: https://pan.baidu.com/s/1dqoLHQzCGqf2Vfeck0obTw 提取码: 5qj4

升级linux kernel 后，需要重新插入 driver ```sh cd rtl8821CU sudo usb_modeswitch -KW -v 0bda -p 1a2b lsusb # 0bda:1a2b ```

经核实吧主atongsa 未通过普通吧主考核。违反《百度贴吧吧主制度》第八章规定http://tieba.baidu.com/tb/system.html#cnt08 ，无法在建设 atongsa吧 内容上、言论导向上发挥应有的模范带头作用。故撤销其吧主管理权限。百度贴吧管理组

亲爱的atongsa吧的吧友们：大家好！ “atongsa”为本吧吧主候选人得票最多者，共计0张真实票数，根据竞选规则，官方最终批准@atongsa，为本吧正式吧主。公示期三天。 吧主上任后，请严格遵守吧主协议 https://tieba.baidu.com/mo/q/newapply/rule?from=task，履行吧主义务，积极投身本吧的发展建设，也请广大吧友进行监督。如出现违规问题，请至贴吧反馈中心进行反馈或者投诉http://tieba.baidu.com/pmc/reportBazhu

小刺猬+七叶树

sudo apt install build-essential git dkms cd ~/Documents/dn/app git clone https://gitee.com/atongsa/rtl8821CU.git cd rtl8821CU chmod +x dkms-install.sh sudo modprobe 8821cu lsusb # 0bda:1a2b reboot sudo usb_modeswitch -KW -v 0bda -p 1a2b

pca <- prcomp(df_g_m_a) dtp <- data.frame('Breeds' = df_g_m$Sample, pca$x[,1:2]) # the first two componets are selected (NB: you can also select 3 for 3D plottings or 3+) pca_p <- ggplot(data = dtp) + geom_point(aes(x = PC1, y = PC2, col = Breeds)) + theme_minimal()

bug: error: dependencies Go.db not installed solve: wget https://mirrors.tuna.tsinghua.edu.cn/bioconductor/packages/3.12/ install.packages("./ur_path/Go.db.tar.gz") ex: the go.db is too big so install from local is good

bug: can't set uid to 0 solve: ```sh chown -R root:root of below files # singularity/etc # singularity/libexec/singularity/bin/starter-suid chmod 4755 starter-suid ```

C lustered R egularly I nterspaced S hort P alindromic R epeats (CRISPR) Type II system Prior to CRISPR/Cas9, genome engineering approaches , like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs), relied upon the use of customizable DNA-binding protein nucleases that required scientists to design and generate a new nuclease-pair for every genomic target

微电子结构在纳米级别的时候物理性质和化学性质会发生质的改变，举个例子，电流通过微型半导体分子的时候会带动分子的构型和键角的改变，从而破坏分子半导性

## install conda install -c bioconda hmmer dnf install hmmer cd /home/atongsa/Documents/apps/proj/denovo/untar/hmmer-3.2.1 ./configure --prefix=/home/atongsa/.local/ # --disable-threads flag to ./configure # make distclean make make check make install (cd easel; make install) ## usage ### option --cpu <n>; defalt 2, HMMER needs <n> +1 cores - master thread handles I/O --enable-mpi; u need openmpi --mpi; hmmbuild scales well up to hundreds of processors ## tutorial hmmbuild globins4.hmm globins4.sto hmmsearch globins4.hmm globins45.fa ## pros nhmmer search DNA query against DNA sequ

- connect systemctl start sshd ip a passwd ssh root@ur_ip - boot mode ls /sys/firmware/efi/efivars - time timedatectl set-ntp true timedatectl status - disk fdisk -l fdisk /dev/sda n n n mkfs.vfat /dev/sda1 mkswap -f /dev/sda2 mkfs.ext4 /dev/sda3 - mount swapon /dev/sda2 mount /dev/sda3 /mnt mkdir /mnt/boot mkdir /mnt/boot/EFI mount /dev/sda1 /mnt/boot/EFI - mirror vim /etc/pacman.d/mirrorlist pacman -Syy - base pacstrap /mnt base base-devel linux linux-firmware genfstab -U /mnt >> /mnt/etc/fstab - change arch-chroot /mnt - time ln -sf /usr/share/zoneinfo/Asia/Shanghai /etc/localtime hwc

2019年诺贝尔生理医学奖 nature diss:

Array programming with NumPy doi.org/10.1038/s41586-020-2649-2

The Needleman–Wunsch algorithm is still widely used for optimal global alignment

gcc 编译器需要4.9及以上； # 更新头文件 export LD_LIBRARY_PATH # 更新编译器 export PATH=gcc5

请各位大佬看看，跑GSEA时为什么会出现这种情况，急求解答

经核实吧主atongsa 未通过普通吧主考核。违反《百度贴吧吧主制度》第八章规定http://tieba.baidu.com/tb/system.html#cnt08 ，无法在建设 atongsa吧 内容上、言论导向上发挥应有的模范带头作用。故撤销其吧主管理权限。百度贴吧管理组

https://conda.github.io/conda-pack/ conda install -c conda-forge conda-pack conda pack -n my_env -o out_name.tar.gz conda pack -p /explicit/path/to/my_env mkdir -p my_env tar -xzf my_env.tar.gz -C my_env source my_env/bin/activate conda-unpack source my_env/bin/deactivate

- for 支付宝扫“福”字 - talk is cheap, below is the code library(ggplot2) df <- data.frame(x=c(0.5), y=c(0.5)) p <- ggplot(df, aes(x=x, y=y)) + geom_point(size=127, color='red', shape=23, bg='red') + theme_void() + annotate("text", x=0.5, y=0.5, label="福", size=64, fontface=7, angle=0) ggsave("chinese_fu.png", p, width = 5, height = 5)

Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells. Propidium iodide is used as a DNA stain in fl

sudo apt-get update sudo apt-get wine winecfg

https://unix.stackexchange.com/questions/202883/create-xkb-confi https://www.mankier.com/1/setxkbmap#-print xhost + setxkbmap -display :0 -print > mykeymap xkbcomp mykeymap :0

ok ithink i find a good place to work the things out

## R; RSpectra - installation of package ‘RSpectra’ had non-zero exit status - ERROR: failed to create lock directory install("RSpectra", INSTALL_opts = c('--no-lock')) unlink("/home/me/src/Rlibs/00LOCK-Rcpp", recursive = TRUE)

matplotlib run in atom editor but i can not plot it solve: set QT_XKB_CONFIG_ROOT environmental variable

- 黑色的刺眼： theme reverse改成白色的 - insert select text # atom-ide-terminal; send select '.platform-linux atom-workspace, atom-text-editor': 'ctrl-enter': 'atom-ide-terminal:insert-selected-text'

- dynamic languages all have - py example class Duck: def quack(self): print("Quaaaaaack!") def feathers(self): print("The duck has white and gray feathers.") class Person: def quack(self): print("The person imitates a duck.") def feathers(self): print("The person takes a feather from the ground and shows it.") def name(self): print("John Smith") def in_the_forest(duck): duck.quack() duck.feathers() def game(): donald = Duck() john = Person() in_the_forest(donald) in_the_forest(john) game()

## debugger-python sudo apt install libssl1.0-dev sudo apt install npm apm install atom-ide-debugger-python

Yang Ziheng hold the R.A. Fisher Chair of Statistical Genetics at UCL he is really a hero

时天旱禁酒，酿者有刑，吏于人家索得酿具，论者欲令与作酒者同罚。 雍与先主游观，见一男女行道，谓先主曰：“彼人欲行淫，何以不缚？“ 先主曰：”卿何以知之？“ 雍对曰：”彼有其具，与欲酿者同。“ 先主大笑，而原欲酿者。

In fact, 62 moonquakes were detected in data recorded between 1972 and 1977

free will is inviolability

diff: With Singularity you can’t become a different user inside a container, the user inside the container is the same user who started the container. Mount namespace (container has own filesystem)

today is a rainny day i like c.k i like code i like sci-fi i dream that a gret sci-fi but i forgot it last night what a pitty

# i remove the conda gio path # normal sudo vim /usr/bin/gvfs-trash #!/bin/bash # GVFS updated and dropped gvfs-trash to gio, but Atom didn't update. /usr/bin/gio trash "$@" sudo chmod +x /usr/bin/gvfs-trash

衡量向量长度： 非负 齐次 三角不等 L1-Norm L2-Norm

Shelley found a creative outlet in writing Shelley went to Scotland to stay with an acquaintance of her father William Baxter and his family 1812 She married poet Percy Bysshe Shelley in 1816 she published her most famous novel, Frankenstein 1818 She wrote several more novels, including Valperga and the science fiction tale The Last Man 1826 Mary Shelley died of brain cancer on February 1, 1851, at age 53, in London, England Mathilde, was finally released in the 1950s

if u have empty location there will be waring and error in the future df2.reindex_like(df1,method='ffill',limit=1) df.reindex(index=[0,2,5], columns=['A', 'C', 'B']) df1.rename(columns={'col1' : 'c1', 'col2' : 'c2'}, index = {0 : 'apple', 1 : 'banana', 2 : 'durian'}) loc for location iloc interger location

diff -Naur old_file new_file > diff_file patch < patchfile.txt tr -d '\r' < dos_file > unix_file echo "secret text" | tr a-zA-Z n-za-mN-ZA-M echo "aaabbbccc" | tr -s ab echo "front" | sed 's_front_back_' echo "front" | sed '2s/front/back/' sed -n '1,5p' distros.txt sed -n '/SUSE/p' distros.txt sed -n '/SUSE/!p' distros.txt =/a/d/i/p/