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那个啥。。关于prion的事情。。。

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我实在是很不愿意开个楼的。。。但是既然那谁谁@郭建仁prince 艾特我了我就来顺手看看我能找到什么资料。。。
神评论镇楼。相关游戏请移步steam搜索goat simulator.
My whole life has been empty. The doctors could not tell me why I was so depressed, and I never knew myself until today. I needed to experience my life as a goat. Goat Simulator has filled the void in my life.
I knew as soon as I hit the truck with my goat that this was what I had been missing. I flew through the air in slow motion, tongue flying around, hitting the edge of the map and rebounding back, landing on (and falling through) the roof of a house. I could not get out, but that did not matter. I was treated with the ability to look down into the house beneath me, seeing the unsuspecting people casually sitting at their computers, oblivious to the sheer terror of the goat above them.
Whether it was playing football with a basketball, swinging lampposts into groups of people or even doing 1080 degree flips, there was no limit to life as a goat. I didn't think it could get any better than this.
I was wrong.
As I jumped and screamed my way around the map, leaving a path of destruction in my wake, I happened upon a golden statue. Curiosity overtook me and I resisted the urge to grab it no longer. My life as a goat changed completely. I had a jetpack.
I knew that this was meant to be, and what my mission from the Great Coffee Stain in the sky was. I was granted the supergoat power of being able to lift people and cars with my tongue. I could survive any impact. I could slow down time with mere thought. Now I could fly. It was time for world domination. Humanity had to be silenced so that the new goat overlords could reign supreme.
All the humans are dead or enslaved. I have won. I am the goat, the goat is me.
Go and goat this game. I kid you not, for mere bucks you can experience a sandbox like no other. Destruction, mayhem, death defying stunts. Seriously, there are more than 921 million goats in the world. Killing the humans was just part of the game. This is a SIMULATOR. Do you understand? A SIMULATOR. This can happen. There are enough goats out there to enslave us and/or kill us.
It is your duty as a human to get this game, so that you may truly know your enemy. An enemy people do not take seriously, an enemy that we know nothing about. Coffee Stain studios have granted us the insight into the true terrifying reality of the impending Goatpocalypse. This is your weapon against the goats. Be prepared, for the goats are coming to get us.


IP属地:上海1楼2014-04-20 03:34回复
    争议内容:
    没那么简单的
    2.朊病毒增殖的可能机理:
    大多学者认为有一编码朊病毒氨基酸序列的基因组,但此DNA不存在于朊病毒中,而是正常哺乳动物基因组的一部分。
    朊病毒的感染可活化或改变这一基因,使之转译出蛋白质。
    亦有人认为朊病毒中存在某种小的核酸片段,它可能是基因活化的扳机。此片段插入到寄主细胞染色体的PrP基因前面,即在此基因转录起始位点之前,插入的核酸片段可作为基因表达的启动子或强化子。如果朊病毒本身只含有蛋白质,PrP本身可能结合到DNA控制PrP基因转录的区域而起到同样的作用。大多数结合到DNA上的蛋白质都趋向于阻遏基因的表达,但一种蛋白质刺激其本身合成的现象并不是没有先例[22]。
    此外,有少数学者认为朊病毒是通过与生物中心法则不同的信息流来增殖的。它可能先由PrP转译成RNA或DNA,然后再合成子代PrP。这一过程需要逆转译酶和逆转录酶,前一种酶还从未被发现过,也可大胆设想朊病毒的氨基酸序列可直接作为模板合成新的蛋白质分子,但这种蛋白质指导的蛋白质合成也从未被发现过[22] 。
    可见,朊病毒增殖的研究不仅对这类特殊病原物是重要的,还牵连到生物中心法则,是一个十分有意义的问题。
    我被民科吧封了,只能在这里和@郭建仁prince 简单讨论讨论。。


    IP属地:上海3楼2014-04-20 03:36
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      我记得朊病毒的机制有以下假说。1是作为构想变化反应的催化剂诱导正常蛋白质错误折叠,2是作为分子伴侣在正常蛋白质未正常折叠的情况下诱导成错误的折叠方式,3是作为调控序列与dna作用,激活/失活其他蛋白质分子的基因,而这些蛋白质可影响prp的折叠。


      来自Android客户端4楼2014-04-20 03:54
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        首先,prion是不含有核酸的,是PrPc蛋白的构象改变产生了PrPsc,后者是TSE的病原体。
        我们来看一下Prion细胞内的分布。PrPc是细胞膜上的糖基磷脂酰肌醇锚蛋白,在脂筏(lipid raft)上与各类生物信号分子进行反应。研究表明,PrPsc的分布于PrPc基本相同。如果PrPsc的形成于细胞转录有关的话,那么PrPsc必然在他处也有分布。基于PrPsc不可溶解这一基本事实,PrPsc是不会到达脂筏上的锚点的。


        IP属地:上海5楼2014-04-20 03:59
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          接下来我们来讨论转化过程发生的区域。通常情况下基于楼上列举的事实和其他实验证据,人们认为PrPc到PrPsc的转化是在细胞膜上进行的,最终结果是PrPsc被转运至细胞内小泡中。所以基本推翻了PrPsc是直接被表达出来的观点。在PrPc的基因序列中存在点多态性,导致PrPsc也存在不同的亚型,其发生的区域亦有所不同。


          IP属地:上海6楼2014-04-20 04:06
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            最后再来看看转化的机制。直接从文献中拷了,懒得翻译了,反正懂的就懂,看不懂的翻译过来你也看不懂=。=The mammalian prion protein consists of ∼210 amino acids arranged in a disordered amino-terminal tail and a globular C-terminal domain composed of three α-helices and a short anti-parallel β-sheet (10, 11). Prion disease develops when a β-sheet-rich prion protein aggregate, known as PrPSc, recruits and templates the conversion of the normal cellular prion protein, PrPC, in an autocatalytic process (12, 13)


            IP属地:上海7楼2014-04-20 04:13
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              综上所述,争议文本中所提出的观点纯属扯淡。


              IP属地:上海8楼2014-04-20 04:14
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                不明觉厉


                IP属地:江苏来自Android客户端9楼2014-04-20 14:22
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                  嗯 我就路过


                  IP属地:上海来自iPhone客户端10楼2014-04-22 01:43
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                    案发地点求@一下


                    IP属地:上海来自iPhone客户端11楼2014-04-22 01:44
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                      PrPc和PrPsc看不懂是啥。。不查了。话说我记得朊病毒不含核酸啊。所以鄙人保守地认为那只是大胆猜测而已吧。。。。等等PrP本身是蛋白?蛋白转译核酸是个啥情况?我的中心法则阿。。。


                      来自iPad12楼2014-04-23 03:16
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