Secondly we simply searched the species name in Genbank and found the numbers of sequence of each genera. these may include both ITS and non-ITS sequences of lichensed and freeliving algae. Then we download the sequences and the information of each sequence is shown in the list. Because Genbank data updated quickly, we have to update this list regularly. The sequences were first downloaded in March and updated in June 1st. There will be another update and new information will be added to the table.
Not all of the sequences can be used in this project, only ITS sequence of lichenlised algae are used. All of the sequence name are recorded in Genbank, it's easy to see which is ITS sequence and which is not. There are several ways to judge whether if the host lichen information is not given in genbank, including finding information in original paper and culture record in the website.
Trebouxia species table and Non-trebouxia table are separate. Trebouxia is the most commom lichen algae, most species of Trebouxia are lichenlised, we have relatively more imformation about Trebouxia lichen algae than non-trebouxia species in Genbank. Further more, it would be quite impossible to blastclust non-trebouxia ITS sequences because they can be varied too much to be grouped together. So, non-trebouxia species ITS sequence will only be a background information, BLASTclust will more based on Trebouxia species.
Not all of the sequences can be used in this project, only ITS sequence of lichenlised algae are used. All of the sequence name are recorded in Genbank, it's easy to see which is ITS sequence and which is not. There are several ways to judge whether if the host lichen information is not given in genbank, including finding information in original paper and culture record in the website.
Trebouxia species table and Non-trebouxia table are separate. Trebouxia is the most commom lichen algae, most species of Trebouxia are lichenlised, we have relatively more imformation about Trebouxia lichen algae than non-trebouxia species in Genbank. Further more, it would be quite impossible to blastclust non-trebouxia ITS sequences because they can be varied too much to be grouped together. So, non-trebouxia species ITS sequence will only be a background information, BLASTclust will more based on Trebouxia species.