具体操作步骤：患者排尿后俯卧位于检查台上，腹部垫一直径10－15cm，长50－6－cm的枕头，将肾推向背侧固定，双臂前伸，头偏向一侧。一般选右肾下级为穿刺点，以穿刺点为中心，消毒背部皮肤，铺无菌巾。无菌B超穿刺探头成像，用1－2%利多卡因局部麻醉。取10cm长心内注射针垂直从穿刺点刺入肾囊，注入少量局麻药物。将穿刺针垂直刺+ROBR6BR2OR52R52R2OROER52R2BRN5RO6R21R54RO4R2OR6@ROER2BR61RN1R2OROER52R2OROCRO1R2CR5ORO6R2CR5ORO5R2@R56R6@R2NRN6RO1R2BRNFR54R2BRNGR5OR2@R6@R55R2BRO6R6OR2ARODROBR2BROAR5BR21R54RO4R2BR53RNDR2OROER52R2OROCRO1R2CR5ORO5R2ARN2ROFR2@R6@R55R2BROOR5GR2@R6NR51R2BROOR56R2ARN4ROGR2CR53R5DR2@RN6ROCR2CR53RO3R2@R53R62R2ARN@R5AR21R54RO4R2CR55R6CR2AROER6DR2OROGROBR2ARNFRNER2AR5GRNCR21R54RO4R2@R65RO5R2BRO3R5DR2BR51R65R2NROGRN1R2BR5GROAR2@R6NR51R2BROOR56R2NRNEROOR2BROOR56R2BROBR6BR2OROER52R2OROCRO1R2BROAROBER21R54RO3DR21R54RO4R2ARO5RNCR2BRO@R56R2@R6NR51R2BROOR56R2NRNEROOR21R54RO4R2BRNOR5FR2AROER6DR2OROGROBR2AR63R6DR2BR5OR5OR2BRNFR54R2BRNGR5OR2DROGROER2AR6DROGR2ARN1R6BR2ARNOR61R2BRNGR6AR2BRO1RNAR2BROOR5GR2OROER52R2@R55ROCR2@R55RO@R21R54RO4R2BR5NR5AR2AR5BRO5R2NRO@RO1R2BROBR5AR2NRNBR51R2BR56R6AR21R54RO4R2BRN4R6OR2OR6@R6DR2BRONRNAR2NRNBRN4R2CR5ORO5R2OR6@ROER2BR61RN1R2ARN4RONBR2CR5OR66R2CR55R6BR2CR5ORO6R2OROER52R2BR5GRO1R2@RNGRODR2BRNGRO2R21R54RO4R2NROGROFR2BROBRONR2NRNBRN4R2DROGROFR2@RNCR5BR2NRNBRN4R2DROGROFR2BROBRO3R2@RNAR65R2ORO3R6@R2BROBRONR2DROGROER2BR6AROER2ARN@R6GR2OROER52R2@R55ROCR2@R55RO@R2BRO1R61R2NRO@RO3R2BR6CRO3R2CR55R6BR2CR5ORO6R2AR63R6BR2NR66R6CR2DROGROER2CR5OROGR2OROOR64ER21R54RO3DR2AR64R6FR2CR5OR56R2BR62RN4R2DROGROEQJRB5RB3Q(

具体操作步骤：患者排尿后俯卧位于检查台上，腹部垫一直径10－15cm，长50－6－cm的枕头，将肾推向背侧固定，双臂前伸，头偏向一侧。一般选右肾下级为穿刺点，以穿刺点为中心，消毒背部皮肤，铺无菌巾。无菌B超穿刺探头成像，用1－2%利多卡因局部麻醉。取10cm长心内注射针垂直从穿刺点刺入肾囊，注入少量局麻药物。将穿刺针垂直刺入达肾囊，观察肾脏上下级随呼吸移动情况，当肾脏下极移到穿刺最佳的位置时，令患者摒气，立即快速将穿刺针刺入肾脏内2－3cm，拔出穿刺针，嘱患者正常呼吸。检查是否取到肾组织，并测量其长度，在解剖镜下观察有5个以上肾小球后，送光镜、电镜、免疫荧光。如无肾组织可重复以上步骤。一般2－3次为宜。