Efficacy of ronidazole for treatment of feline Tritrichomonas foetusinfection.
罗硝唑治疗猫胎毛滴虫感染的疗效观察。
OBJECTIVES:
To determine the efficacy of ronidazole (RDZ), tinidazole (TDZ),and metronidazole (MDZ) against Tritrichomonas foetus in vitro and of RDZ fortreatment of feline naturally occurring or experimentally induced T. foetusinfection.
目的研究罗硝唑(RDZ)、替硝唑(TDZ)和甲硝唑(MDZ)对体外培养的胎毛滴虫和RDZ治疗猫自然发生或实验诱导的T.胎毛滴虫感染的疗效。
ANIMALS:
A cat naturally infected with T. foetusinfection and diarrhea. Ten specific-pathogen-free (SPF) kittens.
试验对象：一只猫自然感染了T.胎毛滴虫感染和腹泻。10只无特定病原体试验级小猫。
PROCEDURE:
RDZ, TDZ, and MDZ were tested for activityagainst 3 different feline isolates of T. foetus in vitro. RDZ then wasadministered to a naturally infected cat at 10 mg/kg 24h for 10 days. SPFkittens were infected orogastrically with feline T. foetus and treated witheither placebo or RDZ (10 mg/kg PO q12h for 14 days). Cats with relapsinginfection or those receiving placebo were treated subsequently with RDZ (either30 or 50 mg/kg PO q12h for 14 days). Feces were examined for T. foetus bydirect microscopy, culture, and polymerase chain reaction (PCR) testing weekly.
罗硝唑(RDZ)、替硝唑(TDZ)和甲硝唑(MDZ) 对3种不同猫胎毛滴虫分离物的体外活性进行了检测。然后以10mg /kg 一天给一只自然感染的猫服用RDZ罗硝唑10天。无特定病原体试验级小猫经口服胎毛滴虫感染，给予安慰剂或罗硝唑RDZ (10mg/kg12h小时，连续14天)治疗。复发感染或接受安慰剂的猫随后接受RDZ治疗(30或50 mg/kg POq12h，持续14天)。每周用直接镜检、培养和聚合酶链反应(PCR)检测粪便中T.胎毛滴虫。
RESULTS:
Both RDZ and TDZ killed T. foetus atconcentrations >0.1 microg/mL in vitro. In the naturally infected cat, RDZabolished diarrhea and T. foetus infection for 85 days after treatment, atwhich time infection and diarrhea relapsed. Retreatment with RDZ eradicateddiarrhea and T. foetus infection for over 407 days. In experimentally inducedinfection, RDZ at 10 mg/kg caused initial improvement, but infection relapsedin all 5 cats 2 to 20 weeks after treatment. At 30 or 50 mg/kg, 10/10 cats werenegative for T. foetus infection for follow-up durations of 21 to 30 weeks aftertreatment.
RDZ罗硝唑和TDZ替硝唑均能在体外以浓度为>0.1微克/mL的条件下杀死胎毛滴虫。10 mg/kg的RDZ引起了最初的改善，但在治疗后2 - 20周，所有5只猫的感染复发。在30或50 mg/kg时，10/10只猫在治疗后21至30周的随访时间内对胎毛滴虫感染呈阴性。
CONCLUSIONS AND CLINICAL RELEVANCE:
Oral administration of RDZ at 30 to 50 mg/kg q12h for 14 daysresolved diarrhea and eradicated infection (on the basis of polymerase chainreaction [PCR] testing) in 1 naturally infected cat and 10 experimentallyinoculated cats receiving a different isolate of T. foetus.
最终结论：口服30 ~ 50mg /kg12h RDZ 14天，1只自然感染的猫和10只
实验接种不同分离株胎毛滴虫的猫，腹泻得到缓解，感染得到根除(基聚合酶链反应[PCR]检测)。
罗硝唑治疗猫胎毛滴虫感染的疗效观察。
OBJECTIVES:
To determine the efficacy of ronidazole (RDZ), tinidazole (TDZ),and metronidazole (MDZ) against Tritrichomonas foetus in vitro and of RDZ fortreatment of feline naturally occurring or experimentally induced T. foetusinfection.
目的研究罗硝唑(RDZ)、替硝唑(TDZ)和甲硝唑(MDZ)对体外培养的胎毛滴虫和RDZ治疗猫自然发生或实验诱导的T.胎毛滴虫感染的疗效。
ANIMALS:
A cat naturally infected with T. foetusinfection and diarrhea. Ten specific-pathogen-free (SPF) kittens.
试验对象：一只猫自然感染了T.胎毛滴虫感染和腹泻。10只无特定病原体试验级小猫。
PROCEDURE:
RDZ, TDZ, and MDZ were tested for activityagainst 3 different feline isolates of T. foetus in vitro. RDZ then wasadministered to a naturally infected cat at 10 mg/kg 24h for 10 days. SPFkittens were infected orogastrically with feline T. foetus and treated witheither placebo or RDZ (10 mg/kg PO q12h for 14 days). Cats with relapsinginfection or those receiving placebo were treated subsequently with RDZ (either30 or 50 mg/kg PO q12h for 14 days). Feces were examined for T. foetus bydirect microscopy, culture, and polymerase chain reaction (PCR) testing weekly.
罗硝唑(RDZ)、替硝唑(TDZ)和甲硝唑(MDZ) 对3种不同猫胎毛滴虫分离物的体外活性进行了检测。然后以10mg /kg 一天给一只自然感染的猫服用RDZ罗硝唑10天。无特定病原体试验级小猫经口服胎毛滴虫感染，给予安慰剂或罗硝唑RDZ (10mg/kg12h小时，连续14天)治疗。复发感染或接受安慰剂的猫随后接受RDZ治疗(30或50 mg/kg POq12h，持续14天)。每周用直接镜检、培养和聚合酶链反应(PCR)检测粪便中T.胎毛滴虫。
RESULTS:
Both RDZ and TDZ killed T. foetus atconcentrations >0.1 microg/mL in vitro. In the naturally infected cat, RDZabolished diarrhea and T. foetus infection for 85 days after treatment, atwhich time infection and diarrhea relapsed. Retreatment with RDZ eradicateddiarrhea and T. foetus infection for over 407 days. In experimentally inducedinfection, RDZ at 10 mg/kg caused initial improvement, but infection relapsedin all 5 cats 2 to 20 weeks after treatment. At 30 or 50 mg/kg, 10/10 cats werenegative for T. foetus infection for follow-up durations of 21 to 30 weeks aftertreatment.
RDZ罗硝唑和TDZ替硝唑均能在体外以浓度为>0.1微克/mL的条件下杀死胎毛滴虫。10 mg/kg的RDZ引起了最初的改善，但在治疗后2 - 20周，所有5只猫的感染复发。在30或50 mg/kg时，10/10只猫在治疗后21至30周的随访时间内对胎毛滴虫感染呈阴性。
CONCLUSIONS AND CLINICAL RELEVANCE:
Oral administration of RDZ at 30 to 50 mg/kg q12h for 14 daysresolved diarrhea and eradicated infection (on the basis of polymerase chainreaction [PCR] testing) in 1 naturally infected cat and 10 experimentallyinoculated cats receiving a different isolate of T. foetus.
最终结论：口服30 ~ 50mg /kg12h RDZ 14天，1只自然感染的猫和10只
实验接种不同分离株胎毛滴虫的猫，腹泻得到缓解，感染得到根除(基聚合酶链反应[PCR]检测)。
