http://ijs.sgmjournals.org/cgi/content/abstract/60/5/1236?maxtoshow=&hits=10&RESULTFORMAT=&author1=+Koichi+Makimura&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT
Int J Syst Evol Microbiol 60 (2010), 1236-1240; DOI 10.1099/ijs.0.016402-0
© 2010 International Union of Microbiological Societies
Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin
Kazuo Satoh1,2, Kenji Ooe3, Hirotoshi Nagayama3 and Koichi Makimura1,4
1 Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
2 Japan Health Sciences Foundation, Chuo-ku, Tokyo, Japan
3 Asahi General Hospital, Asahi, Chiba, Japan
4 Genome Research Center, Graduate School of Medicine and Faculty of Medicine, Teikyo University, Hachioji, Tokyo, Japan
Correspondence
Koichi Makimura
makimura@main.teikyo-u.ac.jp
A strain of a novel pathogenic, achlorophyllic alga belonging to the genus Prototheca was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxonomic studies indicated that this strain represents a novel species with a close phylogenetic relationship to Prototheca wickerhamii and Auxenochlorella protothecoides. This strain grew well at 28–30 °C, showed slow and weak growth at 37 °C, and no growth at 40 °C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), L-arabinose and soluble starch as a carbon source. The taxonomic description of Prototheca cutis sp. nov. is proposed (type strain JCM 15793T =CBS 11262T =DSM 22084T) as a pathogen of dermatitis.
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The GenBank/EMBL/DDBJ accession number for the 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences of strain JCM 15793T are AB470468 and AB470469, respectively.
Larger versions of Figures 1 and 2, and a phylogenetic tree based on 26S rRNA gene D1/D2 domain sequences are available with the online version of this paper.
Int J Syst Evol Microbiol 60 (2010), 1236-1240; DOI 10.1099/ijs.0.016402-0
© 2010 International Union of Microbiological Societies
Prototheca cutis sp. nov., a newly discovered pathogen of protothecosis isolated from inflamed human skin
Kazuo Satoh1,2, Kenji Ooe3, Hirotoshi Nagayama3 and Koichi Makimura1,4
1 Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
2 Japan Health Sciences Foundation, Chuo-ku, Tokyo, Japan
3 Asahi General Hospital, Asahi, Chiba, Japan
4 Genome Research Center, Graduate School of Medicine and Faculty of Medicine, Teikyo University, Hachioji, Tokyo, Japan
Correspondence
Koichi Makimura
makimura@main.teikyo-u.ac.jp
A strain of a novel pathogenic, achlorophyllic alga belonging to the genus Prototheca was isolated from the inflamed skin of a patient with protothecosis in a Japanese hospital. The pathogen was detected and isolated in biopsy specimens by histopathology and culture-based examination. Analyses of the nuclear 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences and chemotaxonomic studies indicated that this strain represents a novel species with a close phylogenetic relationship to Prototheca wickerhamii and Auxenochlorella protothecoides. This strain grew well at 28–30 °C, showed slow and weak growth at 37 °C, and no growth at 40 °C. This strain grew in vitamin-free medium and assimilated acetate (pH 5.1), L-arabinose and soluble starch as a carbon source. The taxonomic description of Prototheca cutis sp. nov. is proposed (type strain JCM 15793T =CBS 11262T =DSM 22084T) as a pathogen of dermatitis.
--------------------------------------------------------------------------------
The GenBank/EMBL/DDBJ accession number for the 18S rDNA gene and 26S rDNA gene D1/D2 domain sequences of strain JCM 15793T are AB470468 and AB470469, respectively.
Larger versions of Figures 1 and 2, and a phylogenetic tree based on 26S rRNA gene D1/D2 domain sequences are available with the online version of this paper.
