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Monitoring genetically modified soybean along the industrial soybean oil extraction and refining
processes by polymerase chain reaction techniques; Joana Costaa, Isabel Mafraa, , Joana S. Amarala, b
and M.B.P.P. Oliveiraa; Food Research International Volume 43, Issue 1, January 2010, Pages 301-306;
Abstract
In the present work, the extraction and detection of DNA along a complete industrial soybean oil
processing chain was described to monitor the presence of Roundup Ready® (RR) soybean. The analysed
samples comprised all the steps prior to industrial oil extraction, namely, raw, cracked, laminated and
expanded seeds, and the defatted flour as a sub-product. The samples collected at the refining unit included
the crude oil, degummed/neutralised, washed, bleached and deodorised oil, as final product. The
amplification of soybean lectin gene by end-point polymerase chain reaction (PCR) was successfully
achieved in all the steps of extraction and refining processes, until the fully refined soybean oil. The
amplification of RR soybean by PCR assays using event-specific primers was also achieved for all the
extraction and refining steps, except for the intermediate steps of refining (neutralisation, washing and
bleaching) possibly due to sample instability. The real-time PCR assays using specific probes confirmed all
the results and proved that it is possible to detect and quantify genetically modified organisms in the fully
refined soybean oil. To our knowledge, this has never been reported before and represents an important
accomplishment regarding the traceability of genetically modified organisms in refined oils.
processes by polymerase chain reaction techniques; Joana Costaa, Isabel Mafraa, , Joana S. Amarala, b
and M.B.P.P. Oliveiraa; Food Research International Volume 43, Issue 1, January 2010, Pages 301-306;
Abstract
In the present work, the extraction and detection of DNA along a complete industrial soybean oil
processing chain was described to monitor the presence of Roundup Ready® (RR) soybean. The analysed
samples comprised all the steps prior to industrial oil extraction, namely, raw, cracked, laminated and
expanded seeds, and the defatted flour as a sub-product. The samples collected at the refining unit included
the crude oil, degummed/neutralised, washed, bleached and deodorised oil, as final product. The
amplification of soybean lectin gene by end-point polymerase chain reaction (PCR) was successfully
achieved in all the steps of extraction and refining processes, until the fully refined soybean oil. The
amplification of RR soybean by PCR assays using event-specific primers was also achieved for all the
extraction and refining steps, except for the intermediate steps of refining (neutralisation, washing and
bleaching) possibly due to sample instability. The real-time PCR assays using specific probes confirmed all
the results and proved that it is possible to detect and quantify genetically modified organisms in the fully
refined soybean oil. To our knowledge, this has never been reported before and represents an important
accomplishment regarding the traceability of genetically modified organisms in refined oils.
